Expression of VP5<sup>*</sup>and VP8<sup>*</sup> of Goup A Rotavirus and Immunological Characteristics

China Biotechnology

2010, Vol. 30

Issue (02): 32-38 DOI:

Expression of VP5^*and VP8^* of Goup A Rotavirus and Immunological Characteristics

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Abstract

Infections of rotaviruses are the primary cause of severe infantile gastroenteritis worldwide. VP4 is one of the major antigenic protein of the virus. VP4 has an essential role during the early interactions of the virus with the cell surface, including receptor binding and cell penetration. Cleavage of the VP4 protein prior to cell attachment by trypsin-like enzymes into two peptide fragments VP5* and VP8* that remain associated with the virus particle enhances its infectivity. In order to further investigate properties of this two proteins, open reading frame (ORF) sequences of VP5* and VP8* proteins derived from the VP4 of rotavirus strain TB-Chen were cloned and expressed and their immunological characteristics were analyzed. The results showed that VP5* and VP8* proteins could be highly expressed in E. coli cells; the expressed recombinant VP5* (rVP5*) and VP8* (rVP8*) could elicit specific antibodies in guinea pigs, these antibodies could recognize the recombinant VP4 derived from TB-Chen strain and the VP4 protein synthesized in Wa infected or SA11 infected MA104 cells. The results indicated that rVP5* and rVP8* have good immunogenicity and anti-rVP5* and anti-rVP8* antibodies have high specificity and cross reactivity.

Key words：轮状病毒 VP5^* VP8^* 重组表达抗原性

Received: 14 September 2009 Published: 26 February 2010

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	Articles by authors
	ZHANG Yan
	WEN Yu-Ling
	WEI Hai-Chao
	LI Chuan-Yi
	FAN Yao-Chun
	CHEN Yuan-Ding

Cite this article:

ZHANG Yan, WEN Yu-Ling, WEI Hai-Chao, LI Chuan-Yi, FAN Yao-Chun, CHEN Yuan-Ding. Expression of VP5^*and VP8^* of Goup A Rotavirus and Immunological Characteristics. China Biotechnology, 2010, 30(02): 32-38.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I02/32

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