|
|
|
| Optimization of Cultivation Conditions for Protease Production from Marine Bacteria by Response Surface Methodology |
| ZHANG Qi1, NING Xi-bin1, ZHANG Ji-lun2 |
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
2. Comprehensive Laboratory of Shanghai Airport Entry-Exit Inspection and Quarantine Bureau, Shanghai 201207, China |
|
|
|
Abstract Minitab 16 software was applied to optimize the conditions for protease production of marine bacteria SE2011. On the basis of single factor experiments, the Plackett-Burman design was utilized to analyze effect factors. Among the eight factors studied, salinity, pH value and volume of medium per flask had significant effects on protease production (P<0.05). The path of steepest ascent was undertaken to approach the optimal region of the protease production. The regression analysis was further investigated by using Box-Behnken design and response surface analysis. By solving regession equation, the highest protease production was obtained at salinity 2.0%, pH 8.0 and volume of medium 23 ml per flask. The protease production reached to 1336.462 U/ml, which increased 82.3% compared to the initial 733.269 U/ml.
|
|
Received: 20 March 2013
Published: 25 August 2013
|
|
|
|
|
[1] Mehta V J, Thumar J T, Singh S P. Production of alkaline protease from an alkaliphilic actinomycete. Bioresource Technol, 2006, 97(14): 1650-1654.
[2] 马永强, 张浩, 杨春华, 等. 地衣芽孢杆菌2709蛋白酶分离纯化研究. 食品科学, 2010, 31(11):141-146. Ma Y Q, Zhang H, Yang C H, et al. Seperation and purication of protease from Bacillus licheniformis 2709. Food Science, 2010, 31(11):141-146.
[3] Deng A, Wu J, Zhang Y, et al. Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001. Bioresour Technol, 2010, 101(18): 7100-7106.
[4] Reddy L V A, Wee Y J, Yun J S, et al. Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches. Bioresour Technol, 2008, 99(7): 2242-2249.
[5] Chang M Y, Tsai G J, Houng J Y. Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enzyme and Microbial Technology, 2006, 38(3-4): 407-414.
[6] Wen W T, Tsou T Y, Liu H L. Response surface optimization of microbial prodigiosin production from Serratia marcescens. Journal of the Taiwan Institute of Chemical Engineers, 2011, 42(2):217-222.
[7] 张祁, 宁喜斌. 上海临港海域产蛋白酶海洋细菌的筛选及鉴定. 食品工业科技, 2013, (3):192-197. Zhang Q, Ning X B. Isolation and identification of protease-producing marine bacteria from Shanghai Lingang sea area. Science and Technology of Food Industry, 2013, (3):192-197.
[8] Lyman J, Fleming R H. Compositon of seawater. Marine Res, 1940, 3:134-146.
[9] 张晓华. 海洋微生物学. 北京: 中国海洋大学出版社,2007.316. Zhang X H. Marine Microbiology. Beijing: China Ocean University Press, 2007.316.
[10] 李丹, 陈丽, 李富超, 等. 一株产低温碱性蛋白酶海洋细菌Pseudoalteromonas flavipulchra HH407的筛选与生长特性. 食品与生物技术学报, 2007, 26(6): 74-80. Li D, Chen L, Li F C. Isolation and physiological characterization of a marine psychrotroph Pseudoalteromonas flacipulchra HH407 producing cold-active alkaline protease. Journal of Food Science and Biotechnology, 2007, 26(6): 74-80.
[11] 中华人民共和国专业标准(SB/T 10317-1999) . 蛋白酶活力测定法. 北京: 中国标准出版社, 1999. Professional Standards of the People's Republic of China (SB/T 10317-1999). Measurement of Protease Activity. Beijing: China Standard Publishing House, 1999. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
