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| Comparison of the Impact on the Transgene Expression Either by Exogenic Bovine Prolactin, or with Growth Hormone |
| YAN Ya-bin1, CAI Qin1,2, CAI Lin-lin1,2, GONG Xiu-li1,2, ZHU Yi-wen1,2, GUAN Yi-hua1,2, HUANG Ying1,2 |
1. Shanghai Children’s Hospital, Shanghai Children's Hospital of Shanghai JiaoTong University, Shanghai Institution of Medical Genetics, Shanghai 200040, China;
2. Key Laboratory of Medical Embryo and Molecular Biology of the Ministry of Health and Shanghai Key Laboratory of Reproduction and Embryo Engineering, Shanghai 200040, China |
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Abstract Objective: Transgenic murine mammary epithelial cells carrying exogenic human transferrin gene, were transfected with either bovine prolactin (bPRL)-expressing plasmid, or human growth hormone (bGH) expressing cassette driven by goat β-casein promoter. Human transferrin expression levels were then compared in order to have a useful information guiding for effectively generation of transgenic big animals. Methods: bPRL-and bGH-expressing plasmids were constructed, respectively, driven by goat-casein promoter (P1A3). Upon transfection them into transgenic murine mammary epithelial primary cells, the cells and the culture supernatants were collected with the passages. hTF mRNA and protein expression levels were analyzed and compared by RT-PCR and Elisa. Results: Both bPRL and bGH could enhance hTF expressing effectively in the transfected mammary epithelial cells evidenced by RT-PCR, the ELISA results showed that both gene products could enhance hTF transgene expression with no statistically significant difference between them. Conclusion: It was reported for the first time that both bPRL and bGH could be used for enhancing transgene expression. Therefore, they could be a good choice used for effectively expressing transgene in mammary gland bioreactor in transgenic cattles.
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Received: 01 April 2013
Published: 25 August 2013
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