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| Tibetan Sheep Mammary Gland Lysozyme: Molecular Cloning, Prokaryotic Expression and Its Antibacterial Activity |
| LI Jian-bo1,2, JIANG Ming-feng1,2, WANG Yong1 |
1. Sichuan Provincial Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, Chengdu 610041, China;
2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China |
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Abstract The full length of LZM cDNA sequence in mammary gland of Tibetan sheep was cloned into pET-32α, generating a recombinant plasmid pET-32α-LZM which was successfully expressed its protein product in Escherichia coli BL21. The agarose diffusion assay showed that recombinant LZM inhibited the growth of Staphylococcus aureus. Sequence comparison and phylogenetic analysis indicated that LZM gene in mammary gland of Tibetan sheep was most similar to goat. Quantitative Real-Time PCR (qRT-PCR) results showed that LZM mRNA had a highest expression level in the mammary gland. In conclusion, it laid a solid foundation for further study.
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Received: 07 April 2013
Published: 25 August 2013
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