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| ECHS1 Involved in Antagonising Apoptosis in HepG2 Cells via the Mitochondrial Pathway |
| ZHU Xiao-san1,2, DAI Yi-chen1, CHEN Zhang-xing1, XIE Jun-pei1, ZENG Wei1, LIN Yuan-yuan1, ZHAO Ben-hua3 |
1. Department of Gastroenterology, Chenggong Hospital, Xiamen University, Xiamen 361003, China;
2. Medical department of Graduate College of Nanchang University, Nanchang 330006, China;
3. Department of Epidemiology and Health Statistics of Preventive Medicine, Xiamen University, Xiamen 361005, China |
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Abstract Objective: To investigate the impact of enoyl-CoA hydratase short chain 1 ( ECHS1) on apoptosis of HepG2 cells. Methods: The ECHS1 interference plasmids were constructed and transfected into HepG2 cells. The stable interference cell lines were screened via puromycin. Interference efficacy was detected by Western blot. Flow cytometry and TUNEL assays studied the apoptosis of HepG2 cells. Apoptosis-related proteins were detected by Western blot. Results: The HepG2 cells with stable ECHS1 gene interference were successfully established. The expression level of ECHS1 protein in HepG2 cells after transfection with ECHS1 siRNA was significantly lower than that in the blank control cells (HepG2 cells without transfection) and the negative control cells (HepG2 cells transfected with pU6 vector) (P< 0.05). Apoptosis ratio of the ECHS1 siRNA group was significant higher than that of negative control group by both Flow cytometry and TUNEL assays (P<0.05). Expressions of p53, Bax and Bid in ECHS1 siRNA group were higher than those in negative control group. Conclusion: ECHS1 may antagonise the apoptosis of HepG2 cells via the mitochondrial pathway.
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Received: 02 May 2013
Published: 25 August 2013
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