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| Preparation and Immunogenicity of Streptococcus Suis Oral Vaccine in Mice |
| ZHANG Qiu-xiang1, HOU Hui-li2, LU Ying2, CHEN Wei1, ZHONG Jin2 |
1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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Abstract To prevent Streptococcus suis infection, new S. suis oral vaccines were constructed. The virulence factors EF and MRP were first expressed in Escherichia coli BL21 (DE3) pLysS and purified by His-tag. Anti-EF and Anti-MRP polyclonal antibody were prepared by injection of EF and MRP proteins into specific pathogen free mice. Then ef and mrp genes were led by constitutive promoter P59 and signal peptide USP45, subcloned into an expression vector pMG36e and then introduced to Lactobacillus casei ATCC 27092. Western blot showed that EF and MRP proteins were both successfully expressed in L. casei. The production of EF increased gradually, while the expression level of MRP reached a peak when the optical density at 600nm of the recombinant culture was 0.8. The recombinant strains were administered to C57BL/6J mice orally and survived in the intestinal tract of mice for at least 2 days. Expression of EF and MRP in the food-grade vehicle lactic acid bacterium could induce specific anti-EF and anti-MRP antibodies, suggesting it might be a new way to the treatment of s. suis.
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Received: 10 December 2012
Published: 25 July 2013
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