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| Cardiac Transfection of AAV9-FrzA Gene Intervene Wnt Signal Pathway in Ischemic Heart Failure Mice |
| SHEN Xin, MA Yi-tong, YANG Yi-ning, LIU Fen, YU Zi-Xiang, CHEN Bang-dang, CHEN You |
| First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China |
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Abstract Objective: To evaluate the feasibility of recombinant adeno-associated virus serotype 9 carrying FrzA Gene transfection to ischemic heart failure mice to intervene Wnt signal pathway. Methods: 130 of male C57BL/6J mice were divided into sham group(n=10), heart failure(HF) group(n=40), HF+rAAV9-GFP group(n=40), HF+rAAV9-FrzA group(n=40). Ligation of left coronary artery to create HF model and echocardiography was performed after 2 weeks. Then, mice were received tail-vein injection of either rAAV9-GFP or rAAV9-FrzA. After 28 days, the mice were used for detecting expression of FrzA, Dvl-1, GSK3β, p-GSK3β and β-catenin by RT-PCR or Western Blot. Result: rAAV9-FrzA was successfully transfected and expressed in mouse heart. Compared with sham group, heart failure could significantly increase the expression of Dvl-1, p-GSK3β and β-catenin(P<0.05); Compared with HF/HF+rAAV9-GFP groups, rAAV9-FrzA could significantly decrease the expression of Dvl-1, p-GSK3β and β-catenin(P<0.05).Conclusion: rAAV9-FrzA could effectively inhibit the Wnt signal pathway in ischemic heart failure mice, which may support a new idea for gene therpy of heart failure.
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Received: 18 December 2012
Published: 25 July 2013
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