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| Identification of Antimicrobial Lipopetides Component Produced by Isolate from Douchi and Its Antimicrobial Properties |
| SUN Li-jun, WANG Ya-ling, LIU Huan-min, XU De-feng, ZHANG Yong-pin, NIE Fang-hong |
| Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, College of Food Science Technology, Guangdong Ocean University, Zhanjiang 524088, China |
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Abstract Objective: The paper report that screening broad-spectrum antimicrobial activity strain from douchi and identification of antimicrobial component of fermented extracts produced by the isolate. Methods: Through physiological and biochemical experiments and 16SrRNA sequencing analysis, the isolate identification was carried out. Analysis of the related antimicrobial substances was carried out by high performance liquid chromatography (HPLC)-electrospray ionization (ESI)-mass spectrometer (MS). Results: Seven antimicrobial activity isolates were isolated from eight douchi samples. The isolate, strain NT-6 with stronger antimicrobial activity, was identified as Bacillus subtilus, which produced three families of antimicrobial lipopeptides, iturins and fengycins and surfactins. Culture filtrate extracts of strain NT-6 strongly inhibited the growth of all Gram-positive bacteria tested except Bacillus subtilus and all Gram-negative ones and all fungi except Fusarium oxysporum f. sp. vasinfectum. And, antimicrobial activity of the extracts was stable under pH 2~12 and 121℃ 30min conditions. Conclusion: Therefore, it shows good application prospects in the food, agriculture, medicine and other fields that the isolate can produce more antimicrobial lipopeptide ingredients, a wide antimicrobial spectrum, and good adaptability.
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Received: 08 August 2012
Published: 25 July 2013
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