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| Establishment of Protocorm-like Bodies of Anoectochilus Formosanus Hayata in Suspension Culture for the Production of Secondary Metabolites |
| LV Zhi-wei1,2, WU Wen-ping2, BI Li-wei1, JIANG Xing-xing2, WANG Ya-ying1 |
1. Xiamen Medical College, Xiamen 361008, China;
2. Research Center of Xiamen Igreen Bio-tech Co. Ltd., Xiamen 361100, China |
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Abstract The growth and secondary metabolites production of Anoectochilus formosanus Hayata PLBs were increased by manipulating various culture factors and variable process. The results indicates that MS basal medium with S-3307 1.0mg/L, 6-BA 0.5mg/L and 3% sucrose was found to be equally suitable for biomass accumulation (214.45g/L FW and 18.23g/L DW). Whereas the accumulation of total flavonoids, total phenolics and polysaccharides (5.43mg/g DW, 2.87mg/g DW and 243.23mg/g DW) was maximized at MS medium (NH4+∶NO3- ratio is 60∶0mmol/L) supplement with S-3307 1.0mg/L, 6-BA 3.0mg/L and 5% sucrose. The growth pattern was also investigated in suspension culture of PLBs. The maximum biomass production (225.98 g/L FW and 18.53 g/L DW), total flavonoids (5.09mg/g DW) and total phenolics (2.04mg/g DW) were obtained after 7 weeks. However, the polysaccharides production reached its peak (229.36mg/g DW) 5 weeks after suspension culture.
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Received: 30 December 2011
Published: 25 May 2012
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| Fund: Xiamen Government Science and Technology Program(3502Z20080012) |
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