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中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (05): 36-42    DOI:
    
Cloning and Expression of O-linked β-N-acetylglucosaminidase Gene from Ostrinia furnacalis
ZHOU Gang, YANG Jun, YANG Qing
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116023,China
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Abstract  

O-GlcNAcylation is a dynamic and reversible process, regulated by two key enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). An insect OGA gene was extracted from the fifth instar larva of the Asian corn borer Ostrinia furnacalis (Guenee).Full length OfOGA gene was 3 541bp, containing 241bp 5'-UTR, 3 165bp coding region and 132bp 3'-UTR.Open reading frame (ORF) of OfOGA was cloned and expressed in E.coli. The recombinant OfOGA consisted of 1 055 amino acids, with a theoretical molecular weight of 118 kDa, but an apparent molecular weight of 130 kDa by SDS-PAGE. Its optimal pH was 5.5, and the optimum temperature was 50℃. Recombinant expression of OfOGA may facilitate the understanding of its role in insect development and growth, and provide potential biological control target.



Key wordsO-GlcNAc      O-GlcNAc transferase      O-GlcNAcase      Recombinant      Expression      Ostrinia furnacalis (Guenee)     
Received: 09 February 2012      Published: 25 May 2012
ZTFLH:  Q78  
Cite this article:

ZHOU Gang, YANG Jun, YANG Qing. Cloning and Expression of O-linked β-N-acetylglucosaminidase Gene from Ostrinia furnacalis. China Biotechnology, 2012, 32(05): 36-42.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I05/36


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