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| The Strategy of Gene Coexpression in Escherichia coli |
| MA Rong1,2, XU Hao3, DING Rui1,2, AO Yong-hua4, ZHANG Li-jun1,2 |
1. College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China;
2. Liaoning Province Research Center of Plant Gene Engineering Technology, Shenyang 110161, China;
3. Jiangsu Huadong Purifying Equipment Co., Ltd, Yangzhou 225800, China;
4. College of Engineering, Shenyang Agricultural University, Shenyang 110161, China |
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Abstract Multi-gene expression in the same host by genetic engineering is an effective approach in studying the regulation of cell development regulation and in manipulating the cellular metabolism of E. coli. There are mainly three types of vectors to coexpress genes in E. coli, including vectors expressing multiple genes from a single transcription unit, vectors expressing multiple genes from multiple transcription units and vectors expressing a single gene.These types of vectors in their construction principles, characteristics, advantages and transformation strategies had been compared, with a major focus on the principle and methodology of using LIC linker in gene cloning with a multi-gene vector.
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Received: 30 November 2010
Published: 25 April 2012
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