The Impact of Sampling on Cytokines Test of Rat Skin Flap Model

China Biotechnology

2012, Vol. 32

Issue (04): 67-71 DOI:

The Impact of Sampling on Cytokines Test of Rat Skin Flap Model

QIN Shi-rui¹, ZHAO Ling¹, XIE Fei¹, MA Xue-mei¹, WANG You-bin²

1. College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100022, China;
2. Peking Union Medical College Hospital, Beijing 100032, China

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Abstract

Aim: Construct rat skin flap model for the research of ischemia/reperfusion injury. The flap samples were harvested from the proximal, middle, and distal part of the viable flap and cytokines expression were detected separately in order to assess whether there were significant differences among the three parts and confirm an equitable way of sampling on ischemia/reperfusion injury of rat skin flap model. Method: Samples were harvested on postoperative day 5. The level of IL-1β, IL-6, TNF-α and EPO were detected to assess the sampling way. Statistical analyses were performed using SPSS. Results:Significant difference in cytokines levels was noted between the two groups. But no statistical difference in cytokines levels in either inter-group. Conclusion:Samples can be harvested at any arbitrary point on a skin flap to stand for the inflammatory level of the whole flap. And it should be sampled from the same position on different flaps in the same experiment. However harvest from the proximal point were encouraged for taking parallel samples.

Key words： Ischemia/reperfusion injury Flap Cytokine Sampling

Received: 09 December 2011 Published: 25 April 2012

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Cite this article:

QIN Shi-rui, ZHAO Ling, XIE Fei, MA Xue-mei, WANG You-bin. The Impact of Sampling on Cytokines Test of Rat Skin Flap Model. China Biotechnology, 2012, 32(04): 67-71.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I04/67

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