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| Expression of Nucleocapsid Protein of Canine Distemper Virus Base on Bac-to-Bac Baculovirus System |
| FENG Pei-ping1,2, ZHANG Lei2, CHAI Xiu-li2, ZHANG Hai-ling2, ZHAO Jian-jun2, HU Bo2, BAI Xue2, GAO Han2, SHAO Xi-qun2, YAN Xi-jun2, ZHAO Quan1, XU Lei3 |
1. Jilin Agricultural University, Changchun 130118, China;
2. Institute of Special Wild Economic Animals and Plants, CAAS, Changchun 130122, China;
3. China Institute of Veterinary Drug Control, Beijing 100081, China |
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Abstract Objective: The nucleocapsid (N) protein of Canine distemper virus(CDV) was expressed base on Bac-to-Bac baculovirus expression system. CDV-N protein was used as a diagnostic antigen for detecting positive serum of CDV. Methods: N gene fragment was amplified by RT-PCR. Recombinant plasmid Bacmid CDV-N transfected into Sf9 cells was builded.CDV-N protein was expressed and shown by Western blot analysis and indirect immunofluorescence. Results:The N protein was correctly expressed. Thirty six serum were tested at anti-dilution 1∶80, respectivly diluted 1∶40(6.3μg/100μl) with CDV-N protein as the best reaction concentration by indirect ELISA, compared to virus neutralizing sensitivity 96.0%, specificity 81.8% and compliance rate 91.7%. Conclusion:The recombinant CDV-N protein was used as antigen in iELISA and shown to react with CDV antisera as a serological tool for the mass screening of CDV antibodies.
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Received: 04 January 2012
Published: 25 April 2012
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Cite this article:
FENG Pei-ping, ZHANG Lei, CHAI Xiu-li, ZHANG Hai-ling, ZHAO Jian-jun, HU Bo, BAI Xue, GAO Han, SHAO Xi-qun, YAN Xi-jun, ZHAO Quan, XU Lei. Expression of Nucleocapsid Protein of Canine Distemper Virus Base on Bac-to-Bac Baculovirus System. China Biotechnology, 2012, 32(04): 60-66.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I04/60
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