中国生物工程杂志

Objective: A comparative study of mice surviving under 8Gy high-dose γ ray irradiation by gavaged Acai berry based herbal clean supplementary blend and Noni fruit powder was reported. It was evaluated for eliminating free radicals, reducing the hematopoietic cell apoptosis and immuneregulation. Methods: C57BL/6 mice were randomly divided into groups of half male and half female, Acai or Noni powder used at the same dose, respectively or combinedly gavage use for 10 days before or/and after ⁶⁰Co-γ irradiation at 8Gy. Firstly in the following 40 days after irradiation the surviving rate was observed; a duplicated test under the same treatments were employed for peripheral white blood cell count, CD4⁺ and CD8⁺ lymphocyte type, reactive oxygen species, as well as bone marrow cell apoptosis analysis. Results: The saline group C57BL/6 mice irradiated by 8Gy all died in the first 16 days (n = 20, the same in other groups), the mortality rate 100%, while among the pre-gavaged groups, the survive rate were Acai plus Noni 10/20, Noni fruit powder group 9/20, Acai group 8/20 in the following 40 days after irradiation. And the survive rate in the groups of gavaged after irradiation were: Acai alone 2/20, Noni alone group 7/20, Acai with Noni group 4/20 in the following 40 days after irradiation(30 days after gavage). Gavaged the fruit powder before and/or after irradiation were found all increased leukocytes in peripheral blood, and decreased bone marrow cells apoptosis, and in all the test groups the Th/Tc ratio was maintained in the normal range compared with the control group. But red blood cells and platelets without significant changes in the data, yet showed no correlation change in reactive oxygen levels. Conclusion: Acai berry and the Noni fruit powder were helpful for mouse anti-radiation and had a preventive effect, and they maintained hematopoietic cellular proliferation, decreased apoptosis under radiation, and maintained Th/Tc immune balance that were contributions to the anti-radiation. It was demonstrated that Acai berry and Noni fruit powder can improve hematopoietic ability, decrease the hematopoietic cell apoptosis, and keep immune resistance to radiation damage. It also showed that taking Herbal Clean Energy Acai berry powder and NoniGIA Noni fruit powder obviously had positive effect for preventing the radiation damage to human health.