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| Preparation and Characterization of Silk Fibroin Treated with Different Calcium-alcohol Solution |
| LI Ling-ling1, ZHOU Wei2, DAI Fang-yin3, MEI Xiao-xiong2, FAN Cheng-qi2, YANG Xia2, WU Yu-zhang2 |
1. Biochemistry Engineering Department, Chongqing Industry & Trade Polytechnic, Chongqing 408000, China;
2. Institute of Immunology, Third Military Medical University, Chongqing 400038, China;
3. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China |
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Abstract The regenerated silk fibroin were obtained by separately prepared from the calcium nitrate tetrahydrate-methanol solution [molar ratio of Ca(NO3)2 4H2O∶methanol =1∶2], calcium nitrate tetrahydrate-ethanol solution (molar ratio of Ca(NO3)2 4H2O∶ethanol =1∶2), calcium chloride-methanol system(molar ratio of CaCl2∶methanol∶H2O= 1∶2∶8), and calcium chloride-ethanol system(molar ratio of CaCl2∶ethanol∶H2O =1∶2∶8). Then these regenerated silk fibroins were dialyzed against distilled water for 2 days. The regenerated silk fibroin was used as substrate to prepare silk fibroin powder by using freeze-drying method. The molecular mass of liquefied silk fibroin was measured by SDS-PAGE. Regenerated fibroin fibers were examined by the scanning electron microscope (SEM). To obtain fine images, the lyophilized samples were coated with gold. Their surface and fracture images were captured. The molecular conformation of regenerated silk fibroin was investigated by FTIR. SDS-PAGE results showed that regenerated silk fibroin treated with Ca(NO3)2 4H2O-methanol and Ca(NO3)2 4H2O-ethanol had lower molecular weight than CaCl2-ethanol-H2O and CaCl2-methanol-H2O. SEM images revealed that the Ca(NO3)2 4H2O-methanol and CaCl2-ethanol solvent system disolved the silk fibroin completely. FTIR results showed that the molecular conformation of regenerated silk fibroin was between with β-sheet and random coil. These provided a ground work in research of silk fibron which was suitable to serve as drug delivery material.
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Received: 28 December 2011
Published: 25 April 2012
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