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中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (04): 22-27    DOI:
    
Carbonic Anhydrase 1 Is Involved in the Process of Bone Formation
ZHENG Ya-bing1, WANG Lin1, CHANG Jian-fang2, WANG Yue-jian1, HAN Jin-xiang1, CHANG Xiao-tian1
1. Provincial Laboratory for Modern Medicine and Technology of Shandong, Research Center for Medicinal Biotechnology, Shandong Academy of Medical Sciences, Jinan 250062, China;
2. Jinan Military General Hospital, Reproduction Medicine Center, Jinan 250062, China
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Abstract  

Objective: Carbonic anhydrase I (CA1) not only enhances the hydration reaction but also promotes the formation of CaCO3, which is an essential step for new bone formation. However, there is no direct data to demonstrate the involvement of CA1 in bio-mineralization in cells and tissues. The aim is to determine the role of CA1 involvement in bio-mineralization and ossification with cultured cells. Methods: HeLa cells were incubated with dexamethasone (DEX), β-glycerophosphate (β-GP) and ascorbic acid (AA) that can induce bio-mineralization under physiological condition. The cellular calcification was determined by Alizarin Red-S staining. The expression of Runx2/cbfa1, a protein marker of ossification, was detected in the process of bone formation by real-time PCR. The CA1 expression was determined by Western blot. Acetazolamide, an anti-carbonic anhydrase drug was also added to the culture to determine the role of CA1 in bio-mineralization and new bone formation. Results: Following the stimulation, HeLa cells produced a great amount of calcium-rich deposits. Increased transcription of Runx2/cbfa1 was also detected in the HeLa cells, indicating that the DEX, β-GP and AA mixture launched the process of bio-mineralization and bone formation in the cells. CA1 has a significantly increased expression during this process. Following treatment of acetazolamide, the expression of CA1 considerably declined, and calcium nodule formation was significantly decreased. Conclusion: The above findings suggested that CA1 participates in bio-mineralization and ossification,and plays an important roles in the process.



Key wordsBio-mineralization      Carbonic anhydrase I (CA1)      Bone formation     
Received: 30 November 2010      Published: 25 April 2012
ZTFLH:  Q819  
Cite this article:

ZHENG Ya-bing, WANG Lin, CHANG Jian-fang, WANG Yue-jian, HAN Jin-xiang, CHANG Xiao-tian. Carbonic Anhydrase 1 Is Involved in the Process of Bone Formation. China Biotechnology, 2012, 32(04): 22-27.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I04/22


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