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| Search for Optimum Substitutive Neutralizing Agent and pH Control Strategy in Fumaric Acid Fermentation by Rhizopus oryzae |
| CHEN Chen, TAI Chao, LI Shuang |
| State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
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Abstract Four different neutralizing agents (CaCO3, Na2CO3, NH3稨2O, NaOH) were chosen to examine the effects of neutralizing agents on fumaric acid fermentation by Rhizopus oryzae ME-F12 which is the mutant of R. oryzae ATCC 20344. It was found that fumaric acid yield and productivity in the fermentation using Na2CO3 as neutralizing agent were the closest to which in the traditional CaCO3 case. Then the effects of different pH values (3.5, 4.5, 5.5, and 6.5) on fumaric acid fermentation using Na2CO3 as neutralizing agent were investigated. Based on the analysis of three kinetic parameters, a two-stage pH control strategy, aimed at achieving high concentration, high yield and high productivity of fumaric acid simultaneously, was proposed. pH was controlled at 5.5 at the first 24 h, and then switched to 4.5 till the end of the fermentation. Finally, the maximum concentration of fumaric acid reached 40.5 g/L with the yield of 0.55 g/g and the productivity of 0.61 g/L/h, which were 8.3%, 10.0% and 17.3% higher than the best result of constant pH control process. Its productivity was even 3.4% higher than which in CaCO3 case. With the internal advantages of reducing power consumption and simplifying downstream processing, using Na2CO3 as neutralizing agent under two-stage pH control strategy successfully take place of CaCO3.
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Received: 13 December 2012
Published: 25 April 2013
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