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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (7): 27-33    DOI: 10.13523/j.cb.20160705
    
Study on Aluminum Resistant Capacity of 14-3-3 Protein from Cryptococcus humicola
LIU Shuai, QIU Jin-kui, NIAN Hong-juan
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
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Abstract  

14-3-3 proteins are a family of conserved proteins that interact with numerous partner proteins in a phospho-specific manner, and can affect the target proteins in a variety of ways. prokaryotic expression vector of 14-3-3 gene was constructed, and the recombinant 14-3-3 protein was purified, then the 14-3-3 antibody was obtained using this recombinant protein. To characterize the aluminum (Al) tolerance of 14-3-3 protein in yeast, a transgenic Saccharomyces cerevisiae containing pYES3/CT-14-3-3 was generated. The results of growth showed that the transgenic yeast grew better than the control strain on plates with 5mmol/L Al. These results suggest that 14-3-3 conferred Al tolerance in yeast by promoting the growth of yeast.



Key wordsAluminum resistance      Cryptococcus humicola      14-3-3 protein     
Received: 12 January 2016      Published: 16 March 2016
ZTFLH:  Q816  
Cite this article:

LIU Shuai, QIU Jin-kui, NIAN Hong-juan. Study on Aluminum Resistant Capacity of 14-3-3 Protein from Cryptococcus humicola. China Biotechnology, 2016, 36(7): 27-33.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160705     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I7/27

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