|
|
|
| Cloning and Expression Analysis of Verticillium wilt Pathogenesis-related Gene StDAHP in Solanum torvum |
| WANG Zhong1,2,XIE Chao1,JUE Deng-wei1,HUANG Le-ping3,HUANG Quan-sheng3,YANG Qing1 |
1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
2.Research Center of Systematic and Evolutionary Botany, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China
3.Xinjiang Key Laboratory of Crop Biotechnology, Urmuqi 830091, China |
|
|
|
Abstract A homologue of DAHP（3-deoxy-D-arabino-heptulosonate 7-phosphate synthase）, designated StDAHP(GU479467), was isolated from Solanum torvum by homology cloning and RACE. Bioinformatics analysis showed that the full length of the cDNA contained an open reading frame of 1683 bp coding a protein of 560 amino acids, corresponding to a 62.53 kDa polypeptide with an isoelectric point of 9.1. Alignment of the amino acid sequence of StDAHP and that of other homologues showed that StDAHP had a high identity with DAHP from S. tuberosum, S. lycopersicum, N. tabacum, and O. sativa). StDAHP protein was more conservative near its N-terminal and C-terminal. Analysis of StDAHP mRNA level by semi-quantity RTPCR showed that its expression was induced by Verticillium dahliae infection.
|
|
Received: 22 February 2010
Published: 12 June 2010
|
|
Corresponding Authors:
Wang Zhong
E-mail: qyang19@njau.edu.cn
|
|
|
| ［1］ 冯东昕, 李宝栋, 马宾生, 等. 嫁接对茄子黄萎病的抗性及某些生物学性状的影响. 中国蔬菜, 2000, (4): 1315. Feng D X, Li B D, Ma B S. et al. China Vegetables, 2000, (4): 1315.
［2］ 周宝利, 姜荷, 赵鑫. 不同砧木嫁接茄子抗黄萎病特性及其与根系分泌物关系. 沈阳农业大学学报, 2001, 32(6): 414417. Zhou B L, Jiang H, Zhao X. Journal of Shenyang Agricultural University, 2001, 32(6): 414417.
［3］ Kawchuk L M, Hachey J, Lynch D R. Development of sequence characterized DNA markets linked to a dominant Verticillium wilt resistance gene in tomato. Genome, 1988, 41: 9195.
［4］ Kawchuk L M, Lynch D R, Hachey J, et al. Identification of a codominant amplified polymorphic DNA marker linked to the Verticillium wilt resistance gene in tomato. Theoretical and Applied Genetics, 1994, 89: 661664.
［5］ Kawchuk L M, Hachey J, Lynch D R, et al. Tomato Ve disease resistance genes encode cell surfacelike receptors. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98: 65116515.
［6］ 汪华, 崔志峰. 莽草酸生物合成途径的调控. 生物技术通报, 2009, 3:5053. Wang H, Cui Z F. Biotechnology Bulletin, 2009, 3:5053.
［7］ Kosuge T, Gilchrist D G. Aromatic amino acid biosynthesis and its regulation. Biochem Plants, 1980, 5:507531.
［8］ Dixon R. Natural products and plant disease resistance. Nature, 2001, 411(14): 843847.
［9］ 欧阳剑, 李家洋. 拟南芥色氨酸与吲哚乙酸生物合成的研究进展. 中国生物工程杂志, 1998, 18(2): 211. Ouyang J, Li J Y. China Biotechnology, 1998, 18(2): 211.
［10］ 徐晓梅, 杨署光. 苯丙氨酸解氨酶研究进展. 安徽农业科学, 2009, 37(31): 1511515119. Xu X M, Yang S G. Journal of Anhui Agricultral Science, 2009, 37(31): 1511515119.
［11］ Grlach J, Rasescke H R, Rentsch D, et al. Temporally distinct accumulation of transcripts encoding enzymes of the prechorismate pathway in elicitortreated, cultured tomato cells. Proc Natl Acad Sci USA, 1995, 92: 31663170.
［12］ Muday G K, Herrmann K M. Wounding induces one of two isoenzymes of 3deoxydarabinoheptulosonate 7phosphate synthase in Solanum tuberosum L. Plant Physiol, 1992, 98: 496500.
［13］ McCue K F, Conn E E. Induction of 3deoxyDarabinoheptulosonate 7phosphate synthase activity by fungal elicitor in cultures of Petroselinum crispum. Proc Natl Acad Sci USA, 1989, 86: 73747377. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
