Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (12): 99-103    DOI:
    
Optimization of Ganodetma lucidum-Astragalus membranaceus Polysaccharide Solid Fermentation Condition by Response Surface Analysis
ZHU Qiang1, XIA Yan-qiu1, CHEN Ai-qin1, XIONG Xiao-hui2
1. College of Marine, Huaihai Institute of Technology, Lianyungang 222005, China;
2. College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 210009, China
Download: HTML   PDF(869KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Single factor test and response surface analysis were used for optimization of Ganodetma lucidum-Astragalus membranaceus polysaccharide solid fermentation. Optimized solid fermentation conditions were as follows: herbs solid fermentation medium, initial pH6.5, inoculum volume 10.16ml/100g, fermentation temperature 30.65℃, relative humidity 59.54%, fermentation period 28d. Under the above optimum conditions, Ganodetma lucidum-Astragalus membranaceus polysaccharide(GAPS) content was up to 9.12 ± 0.09mg/g and increased by 13.15% than unoptimization(8.06±0.07 mg/g), which provided a theoretical experimental basis for pharmacodynamics research and industrialization development.

Key words： Ganoderma lucidum      Astragalus membranaceus      Ganodetma lucidum-Astragalus membranaceus      polysaccharide(GAPS)      Solidfermentation(SF)     
Received: 01 September 2011      Published: 25 December 2011
ZTFLH:  Q935  
Corresponding Authors: 4     E-mail: xxh.mymr@yahoo.com.cn
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Cite this article:

ZHU Qiang, XIA Yan-qiu, CHEN Ai-qin, XIONG Xiao-hui. Optimization of Ganodetma lucidum-Astragalus membranaceus Polysaccharide Solid Fermentation Condition by Response Surface Analysis. China Biotechnology, 2011, 31(12): 99-103.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I12/99


[1] 庄毅. 药用真菌新型(双向性)固体发酵工程. 中国食用菌,2002,21(4):3-6. Zhuang Y. Edible Fungi of China,2002,21(4):3-6.

[2] 庄 毅,池玉梅,陈慎宝,等.药用真菌新型固体发酵工程与槐芪菌质-F的研制. 中国药学杂志, 2004, 39(3): 175-178. Zhuang Y, Chi Y M, Chen S B, et al. Chinese Pharmaceutical Journal, 2004, 39(3): 175-178.

[3] 阮鸣. 不同药(食)用真菌固体发酵对黄芪中黄芪甲苷的影响. 中草药,2011,42(7):1421-1424. Ruan M. Chinese Traditional and Herbal Drugs, 2011,42(7):1421-1424.

[4] 魏巍. 灵芝-黄芪液体发酵条件优化及缓解体力疲劳功能研究. 成都: 四川农业大学, 2007.06. Wei W. The study on fermentation conditionand relieving physical fatigue funcation of ganoderma-stragalus.Chengdu:Sichuan Agricultural University, 2007.06.

[5] 唐宁,张敦林,张李阳. 芝芪菌质多糖的制备及其增强免疫活性的初步研究.中国预防兽医学报,2010,32(1):68-70. Tang N, Zhang, D L,Zhang L Y. Chinese Journal of Preventive Veterinary Medicine, 2010,32(1):68-70.

[6] 霍光明,张李阳,周业飞,等.芝芪菌质多糖对AA肉鸡生长、免疫和肉品质的影响. 扬州大学学报(农业与生命科学版), 2010,31(3):39-43. Huo G M, Zhang L Y, Zhou Y F,et al. Journal of Yangzhou University (Agricultural and Life Science Edition), 2010,31(3):39-43.

[7]
[JP3]张敦林,张李阳,唐宁,等.芪菌质多糖对CEF的增殖和抵抗NDV感染的研究.安徽农业科学, 2008,36(20):8604-8605,8614. Zhang D L, Zhang L Y, Tang N, et al. Journal of Anhui Agr Sci, 2008,36(20): 8604-8605,8614.

[8] 张雅雯,唐 宁,饶玉鹏,等.芝芪菌质多糖的分离、纯化及体外抗肿瘤活性初步研究.安徽农学通报,2009, 15(13):40-41. Zhang Y W,Tang N,Rao X P, et al. Anhui Agri Sci Bull, 2009, 15(13) :40-41.

[9] 霍光明,张李阳,张永江,等.芝芪菌质对罗氏沼虾生长和免疫及肌苷酸含量的影响.水生态学杂志,2009,12(6):85-88. Huo G M,Zhang L Y, Zhang Y J, et al. Journal of Hydroecology, 2009,12(6):85-88.

[10] 朱强,夏艳秋,董克克,等.响应面法优化灵芝药性固体发酵培养基. 中国生物工程杂志,2010,30(9):75-79. Zhu Q, Xia Y Q, Dong K K, et al. China Biotechnology, 2010,30(9):75-79.

[11] 黎晶晶,徐格非.苯酚-硫酸法测定灵芝多糖含量的研究. 杭州化工,2008,38(1):23-26.
[1] SHU Jiang, JIA Yan-Qiu, DONG Ke-Ke, YANG Cong-Fa, HONG Zhi-Jun. Optimization of Ganoderma Lucidum Medicinal Solid Fermentation Medium by Response Surface Test[J]. China Biotechnology, 2010, 30(09): 75-79.
主管：中国科学院 　主办：中国科学院文献情报中心 　中国生物技术发展中心 　中国生物工程学会