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| Construction of a Lentivirus Vector Expressing Three Reporter Genes and Its Experimental Study |
| WANG Li-xian, WANG Dong-yang, LI Xing, XIA Hai-bin |
| Laboratory of Gene Therapy, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China |
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Abstract Lentiviral is a genus of retroviridae, which has minor immune response and can deliver and express large exogenous gene fragment for longer time. Therefore, it is widely used as an ideal vector for delivering the target gene. One type of lentivirus vector containing three types of reporter gene, e.g. mCherry, luciferase and eGFP was constructed. Mcherry and eGFP can be used for cell line screening in vitro because of its intuitive imaging, while luciferase can be used for detecting the growth and metastasis of the tumor cells on animal models in vivo sensitively. The constructed vector was transfected to the malignant glioblastoma U87MG successfully, and the corresponding animal model was developed on nude mice by planting the transfected cells. It provided a model for the evaluation of therapeutic effect on glioblastoma in vivo, and demonstrated a reference way for other tumor cell lines and models which carried reporter genes.
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Received: 09 August 2011
Published: 25 December 2011
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