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| Effect of purA Gene Overexpression on Adenosine Accumulation |
| ZHANG Yang, DU Shan-shan, XIE Xi-xan, XU Qing-yang, CHEN Ning |
| School of Biological Engineering, Tianjin University of Science & Technology, Tianjin 300457, China |
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Abstract Effect of purA gene overexpression on adenosine accumulation was studied in industrial strain Bacillus subtilis XGL(Xan-+Deam-+8-AGr+SGr). PurA gene was cloned from XGL's chromosome by PCR and ligated into the shuttle vector pBE43 which contained P43 promoter. Recombinant plasmid pBE43∷purA was transformed into XGL by electroporation. Reverse transcription PCR and quantitative PCR were used for measuring the transcription of purA. Fermentation parameters were determined in 5 L fermenter. Expression analysis showed that the purA in XGL-SY expressed as 9 times many as that in XGL. Cell growth was dragged a little. However, the production of adenosine was 10.8% higher than before. In conclusion, the overexpression of purA can promote further accumulation of adenosine, which made a foundation to further genetic engineering strain construction.
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Received: 20 September 2011
Published: 25 December 2011
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| Fund: 3 |
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Corresponding Authors:
3
E-mail: ningch@tust.edu.cn
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