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| Identification Domains of PINK1 Interacting with α-Synuclein |
| FU Yue-jiao, DUAN Chun-li, GONG Pu-sheng, JIA Huan-zhen, ZHANG ian-liang, ZHAO Chun-li, LU Ling-ling, ZHAO Huan-ying, YUAN Xing-xing, YANG Hui |
| Beijing Institute for Neuroscience, Capital Medical University, Beijing Center of Neural Regeneration and Repair, Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100069, China |
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Abstract Objective: To identify domains of PINK1 interacting with α-synuclein. Methods: HEK293T cells were transfected with plasmid encoding human pCMV-Myc-α-synuclein together with human 3xFlag-tagged wild type PINK1 or several deletion mutants(G309D;ΔN35; ΔC145; 156~509; Δ156~581;). After detecting the protein expression by Western blot, the functional domains of hPINK1 interacting with human α-synuclein were detected by co-immunoprecipitation. The co-localization of different domains of hPINK1 and human α-synuclein was detected by immunocytochemistry. Result: Co-IP and immunocytochemistry have proved that the PINK1 kinase domain interacted with α-synuclein. Conclusion: The kinase domain of PINK1 interacting with α-synuclein.
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Received: 26 July 2011
Published: 25 December 2011
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Cite this article:
FU Yue-jiao, DUAN Chun-li, GONG Pu-sheng, JIA Huan-zhen, ZHANG ian-liang, ZHAO Chun-li, LU Ling-ling, ZHAO Huan-ying, YUAN Xing-xing, YANG Hui. Identification Domains of PINK1 Interacting with α-Synuclein. China Biotechnology, 2011, 31(12): 10-14.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I12/10
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