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中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (06): 1-6    DOI: 10.13523/j.cb.20140601
    
Expression and Purification of SidK-VatA Complex
XU An-bi1,2, HUANG Lai-qiang1,2
1. School of Life Sciences, Tsinghua University, Beijing 100084, China;
2. The Shenzhen Key Laboratory of Gene and Antibody Therapy, Division of Life & Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
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Abstract  

legionella makes incursions into host cell by employed a conserved mechanism to form a specific, replication-permissive compartment, the "Legionella-containing vacuole" (LCV), and translocates "effector" proteins into host cells for survival in the host cell. SidK is an effector from legionella, it directly bind the VatA subunit that is responsible for hydrolyzing ATP, leading to defects in LCV acidification and avoiding of lysosomal killing of LCV. The SidK protein was expressed in E.coli, the VatA protein was expressed in insect cell, and the interaction of SidK and VatA identified. Finally, the stable SidK-VatA complex was got. This is important for further study the structure of SidK-VatA complex.



Key wordsEffector protein      Pathogene      Host cell      Complex     
Received: 21 April 2014      Published: 25 June 2014
ZTFLH:  Q786  
Cite this article:

XU An-bi, HUANG Lai-qiang. Expression and Purification of SidK-VatA Complex. China Biotechnology, 2014, 34(06): 1-6.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140601     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I06/1


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