|
|
Advance of Large-scale Animal Cell Culture Technology |
MEI Jian-guo1,2, ZHUANG Jin-qiu1,2, WANG Jin-liang2, WANG Yu-mao2, DING Zhuang1, SHEN Zhi-qiang2 |
1. College of Animal Science and Veterinary Medicine, Jilin University, Jilin 130062, China; 2. Shandong Binzhou Animal Science & Veterinary Medicine Insitute, Binzhou 256600, China |
|
|
Abstract In recent years, large-scale animal cell culture technology in the field of biotechnology as one of the most talked about hot spots, have been widely used in the process of the biomedical research and production. Large-scale animal cell culture technology platform based on bio-reactor technology is being built up gradually and becoming increasingly mature, which will become the powerful tools promoting bio-pharmaceutical industry rapid development. Focusing on the application level of this technology and its recent progress,the differences among the different cell culture technology are analyzed for finding its future direction.
|
Received: 12 December 2011
Published: 25 July 2012
|
|
|
|
[1] 陈志南,杨向民. 基于抗体等重组蛋白表达产品的哺乳动物细胞大规模发酵技术. 中国医药生物技术,2009,4(5):325-328. Chen ZH N, Yang X M.The large scale fermentation technology based on the antibody and recombinant protein expression products in mammalian cell.Chin Med Biotechnol, 2009,4(5):325-328. [2] Doel T R. FMD vaccines. Virus Research,2003,91(1):81-99. [3] 魏明旺,张淑香.动物细胞大规模培养的主流技术.生物产业技术,2009,4:85-89. Wei M W,Zhang S X. Mainstream technology for large-scale cultivation of animal cells.Biotechnology & Business, 2009,4:85-89. [4] 吴方丽,金伟波,王保莉,等. 动物细胞大规模培养技术研究进展. 仲恺农业技术学院学报,2005,18(3):64-70. Wu F L, Jin W B, Wang B L, et al. Research advance in large-scale culture of animal cells. Journal of Zhongkai University of Agriculture and Technology, 2005,18(3):64-70. [5] Leigh N P,Paul W S. Scalability of a Disposable Bioreactor from 25L -500L Run in Perfusion Mode with a CHO-Based Cell Line: A Tech Review. BioProcessing Journal,2004,3(4):51-56. [6] 张嗣良,张恂,唐寅,等. 发展我国大规模细胞培养生物反应器装备制造业.中国生物工程杂志,2005,25(7):1-8. Zhang S L, Zhang X, Tang Y, et al. Advance of the bioreactor equipment manufactures in China. China Biotechnology, 2005, 25(7):1-8. [7] 王晓南,王丽环,高 鹏. 动物细胞无血清培养基的优势、特点与实验研究.亚太传统医学,2009,5(2):21-23. Wang X N,Wang L H,GAO P.The advantages, characteristics and experimental study for Animal cells in serum-free culture medium.Asia-Pacific Traditional Medical, 2009,5(2):21-23. [8] 梅建国,庄金秋,沈志强. 动物细胞无血清培养技术研究进展.生物技术,2010,20(3):87-89. Mei J G, Zhuang J Q, Shen Z Q. Advances in research on serum-free culture technology of animal cell. Biotechnology, 2010,20(3):87-89. [9] Pratibhasri A V,Pratibhasri A V, Martin A J, et al. A unique human chorionic gonadotropin antagonist suppresses ovarian hyperstimulation syndrome in rats. Endocrinology, 2009, 150 (8): 3807-3814. [10] 刘兴茂,刘红,叶玲玲,等. CHO工程细胞(11G-S)悬浮培养的无血清培养基的设计. 生物工程学报,2010,26(8):1116-1122. Liu X M, Liu H, Ye L L, et al. Serum-free medium for suspension culture of recombinant Chinese hamster ovary(11G-S)cells. Chin J Biotech, 2010, 26(8):1116-1122. [11] 黄锭,赵亮,谭文松. 犬肾细胞MDCK无血清贴壁及单细胞悬浮培养.生物工程学报,2011,27(4): 645-652. Huang D, Zhao L, Tan W S. Adherent and single-cell suspension culture of Madin-Darby canine kidney cells in serum-free medium. Chin J Biotech, 2011, 27(4): 645-652. [12] Olga O, Noreen T A, Yair A. The chaperone activity of GRP94 toward Insulin-like growth factor II is necessary for the stress response to serum deprivation. Mol Biol Cell,2009,20(6):1855-1864. [13] 余阗. 哺乳动物细胞适应无血清培养的改造.科技资讯,2007, (22):5. Yu T. The transformation of mammalian cells adapted to serum-free culture.Science and Technology Information, 2007,(22):5. [14] 过琴媛,王辉,沈心亮. 微载体培养动物细胞技术的研究进展.微生物学免疫学进展,2007,35(1):73-75. Guo Q Y,Wang H,Shen X L.The technology progress of microcarrier culture in animal cell.Microbiology Immunology,2007,35(1):73-75. [15] 梅建国,林初文,刘吉山,等. 纳米微球在生物医药领域的应用.材料导报,2011,25(2):30-33. Mei J G, Lin C W, Liu J S, et al. The application of microbeads& nanoparticles in the biomedical Field. Materials Review, 2011,25(2):30-33. [16] 张永欣,刘馨,张光明,等. Vero细胞微载体培养技术规模化培养轮状病毒.中国生物制品学杂志,2008,21(4):336-348. Zhang Y X, Liu X, Zhang G M, et al. Large scale culture of rotavirus by vero cell microcarrier technique. Chin J Biologicals, 2008, 21(4):336-348. [17] Hu X W, Gao L H, Li Z H, et al. Increased production of recombinant prourokinase with porou microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor. High Technology Letters, 2006, 12(3):311-317. [18] 汤强,周燕,陈飞,等.利用大孔微载体体外制备工程化组织.生物工程学报,2008,24(1):74-82. Tang Q, Zhou Y, Chen F, et al. Preparing engneered tissues in vitro by macroporous microcarriers. Chin J Biotech, 2008, 24(1):74-82. [19] 刘轶,朱国强.动物细胞培养及微载体技术研究进展.吉林农业大学学报,2007,29(2):203-206. Liu Y, Zhu G Q. Animal cell cultivation and progress in technology of microcarrier. Journal of Jilin Agricultural University, 2007, 29(2): 203-206. [20] Ball P, Simpson D. Improving process economics when developing a vaccine portfolio. Bioprocessing Journal,2010, 9(1):13-21. [21] 梅建国,沈志强,庄金秋.30L生物反应器培养重组CHO细胞生产重组人组织型纤溶酶原激活剂.中国生物制品学杂志,2010,23(4):428-430. Mei J G, Shen Z Q, Zhuang J Q. Production of rt-PA by perfusion culture of recombinant CHO cells in 30L packed bed bioreactor. Chin J Biologicals, 2010, 23(4):428-430. [22] 龚伟,方学平,刘燕,等.用填充床生物反应器连续灌流培养CHO细胞生产HBsAg.中国生物工程杂志,2004,24(7):82-84. Gong W, Fang X P, Liu Y, et al. Continuously perfused cultivation of genetically-engineered CHO cells producing HBsAg in a packed bed bioreactor. China Biotechnology, 2004, 24(7):82-84. [23] 陈文庆,王建超,刘华杰,等.悬浮培养工艺与转瓶培养工艺的比较分析.中国兽药杂志,2010,44(10):37-41. Chen W Q, Wang J C, Liu H J, et al. Comparison of suspension culture technology and process with roller bottle. Chinese Journal of Veterinary Drug, 2010, 44(10):37-41. [24] 张嗣良.我国生物反应器制造水平亟待提高.中国医药报,2006,83:8. Zhang S L. Our bioreactor manufacturing level needs to be improved urgently. Chinese Medicine News,2006,83:8. [25] 唐江伟,吴振强.新型生物反应器结构研究进展.中国生物工程杂志,2007,27(5):146-152. Tang J W, Wu Z Q. Advances in bioreactor structure innovation and related studies. China Biotechnology, 2007, 27(5):146-152. [26] 张韧,秦玉明,陈文庆,等.悬浮培养技术在生物制药中的应用和展望.中国兽药杂志,2011,45(3):56-60. Zhang R, Qin Y M, Chen W Q, et al. Applications and prospects of cell suspension culture in the biopharmaceutical industry. Chinese Journal of Veterinary Drug, 2011, 45(3):56-60. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|