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Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells |
WANG Xiao-xia1,2, LU Na1, LIU Zhi-rong1, XIE Jun1, WANG Hui-zhen1, NIU Bo1, PEI Yi2 |
1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China; 2. Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China |
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Abstract Objective:To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells. Methods:The Aurora-A expressing plasmid was transfected using LipofectamineTM 2000 and the identification of transfected clones was confirmed by Western blot analysis and RT-PCR. The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay. Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis. Results:Aurora-A overexpressing transfectant clone was established, which increased cell invasion as well as protein expression and activity of MMP-9. Conclusion:The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.
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Received: 31 January 2012
Published: 25 July 2012
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