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中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (03): 53-58    DOI:
    
The Inhibitory Effect of Essential Oil from Citrus reticulate Blanco and Their Main Pure Compounds on Penicillium
WANG Hua, TAO Neng-guo, WANG Chang-feng, FAN Feng
College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China
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Abstract  

The essential oil of C. reticulate Blanco was extracted by hydro-distillation and analyzed by GC-MS. Forty six components were identified. The major components were limonene (57.67%), followed by β-linalool (5.36%), 2-carene (4.47%), β-pinene (4.31%), γ-terpinene (4.08%), α-pinene (3.27%), Cyclobutane,1,2-bis(1-methylethenyl)-(2.87%), β-myrcene (2.77%), α-thujene (2.40%), β-phellandrene (2.24%), decanal(1.80%) and citronellal (1.49%) etc. The effects of C. reticulate essential oil and pure compounds in varying concentrations on the growth of Penicillium sclerotiorum by poisoned food technique and liquid culture were also elucidated. Results showed that C. reticulate essential oil has significant inhibitory effect on the growth of P. sclerotiorum in varying concentrations, with the increasing concentration positively correlated with inhibitory efficiency, and 20μl/ml of the essential oil can inhibit the growth of P. sclerotiorum absolutely. Results by poisoned food technique showed that the antifungal activity of the essential oil at a concentration of 20μl/ml became weaker with time progressed. The study of seven pure compounds against P. sclerotiorum by poisoned food technique indicated that the growth of this strain was effectively inhibited by 0.04μl/ml citral and 1.07μl/ml β-linalool, while no obvious inhibition effect was observed by the remaining pure compounds. The present study suggested that the significant inhibitory effect of Ponkan essential oil on the growth of P. sclerotiorum could be attributed a lot to citral and β-linalool in it.



Key wordsEssential oil      Penicillium sclerotiorum      GC-MS      Inhibitory effect     
Received: 20 July 2011      Published: 25 March 2012
ZTFLH:  Q949.96  
Cite this article:

WANG Hua, TAO Neng-guo, WANG Chang-feng, FAN Feng. The Inhibitory Effect of Essential Oil from Citrus reticulate Blanco and Their Main Pure Compounds on Penicillium. China Biotechnology, 2012, 32(03): 53-58.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I03/53


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