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Establishment of Bone Marrow Mesenchymal Stem Cells Expressing Neurotrophic Factor Neurturin and Activity Detection in Vitro |
WANG Wan-pu, SUN Mao-sheng, LI Hong-jun, XIE Tian-hong, YAN Min, ZHOU Yan, YIN Na |
Laboratory of Biomolecular, Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Medical Biology, Kunming 650118, China |
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Abstract Study the protection and repairation of damaged neurons which leads to neurodegenerative diseases, neurotrophic factor Neurturin gene was transfected into Rhesus bone marrow mesenchymal stem cells (rMSC) by recombinant adenovirus. The NTN gene could be transcribed and expressed in cytoplasm of rMSC and be secreted which was detected by RT-PCR、immunofluorescence、Western blotting, and then, the culture of Chicken embryonic dorsal root ganglion test and the culture of Fetal rats midbrain dopaminergic neurons test had indicated that the NTN protein had good biological activity in vitro. The results gave a new method for stem cell transplantation to treat the neurodegenerative diseases caused by neurons damage.
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Received: 09 October 2011
Published: 25 March 2012
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