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中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (02): 123-127    DOI:
    
Methods of Construction and Identification of the Bait Vector in Yeast Two-Hybrid System
LI Yao-feng1,2, ZHANG Nan-yang1, ZHAO Yong-ju1,2
1. College of Animal Science and Technology, Southwest University, Chongqing 400716, China;
2. Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Chongqing 400716, China
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Abstract  

The yeast two-hybrid system was one of conventional methods to probe of the protein-protein interactions. This model which was first developed by Fields and Song in 1998, explored the interactions between cellular proteins in vivo. Many researchers favoured this method in the study of protein-protein and protein-DNA interactions because of its high sensitivity. The development of the construction of the bait protein vector in yeast two-hybrid system, its self-activation and toxic to the host yeast were summarized.



Key wordsYeast two-hybrid system      Bait vector      Bait protein      Self-activation test     
Received: 06 December 2011      Published: 25 February 2012
ZTFLH:  Q819  
Cite this article:

LI Yao-feng, ZHANG Nan-yang, ZHAO Yong-ju. Methods of Construction and Identification of the Bait Vector in Yeast Two-Hybrid System. China Biotechnology, 2012, 32(02): 123-127.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I02/123


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