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Effects of Adenoviral E1B-19K Gene Overexpression on the Growth and Metabolism of HEK293 Cells |
YE Ling-ling, LIU Hong, LI Shi-chong, YANG Zhen-xi, LIU Xing-mao, WANG Qi-wei, CHEN Zhao-lie |
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
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Abstract Apoptosis plays an important role in limiting viable cell density and production performance in large-scale animal cell culture. Overexpression of anti-apoptotic genes is one of the most common strategies used to improve apoptosis resistance of industrial cells. HEK293 cells with adenoviral anti-apoptotic E1B-19K gene were transfected and picked out several representative clones. These clones were further investigated in the apoptotic percentages under various inducing culture conditions and cell growth and metabolism under normal culture condition. The results showed that the overexpression of E1B-19K in HEK293 cells, endowed cells anti-apoptotic ability by decreasing cell death by 60%~80% under inducing culture conditions with reduced glucose, low serum or without glutamine, delayed cells' entry into decay phase by 2 d under normal culture conditions; and had no significant affect on cell metabolisms of glucose, lactate and glutamine. The above results indicate that the overexpression of E1B-19K is a promising strategy to reduce cell death in mammalian cell culture.
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Received: 05 December 2011
Published: 25 February 2012
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