Overexpression of PINK1 in C57BL/6 Mice Striatum May Alleviate Injury Caused by Rotenone

China Biotechnology

2012, Vol. 32

Issue (02): 33-38 DOI:

Overexpression of PINK1 in C57BL/6 Mice Striatum May Alleviate Injury Caused by Rotenone

GONG Pu-sheng, ZHANG Jian-liang, FU Yue-jiao, JIA Huan-zhen, ZHAO Chun-li, LU Ling-ling, DUAN Chun-li, YANG Hui

Beijing Institute for Neuroscience, Capital Medical University, Beijing Center of Neural Regeneration and Repair, Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100069, China

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Abstract

Background: The etiology of Parkinson's disease(PD) involves genetic, environmental, endogenous neurotoxins and other factors. But the current weight of evidence strongly suggests that environmental neurotoxins may play an important role in the etiology of PD. Rotenone is a classical mitochondrial complex-I inhibitor and is the most potent member of the rotenoids, a family of isoflavonoids extracted from Leguminosae plants. More recently, chronic intraperitoneal injection of rotenone has been shown to cause dosedependent dopamine (DA) loss, reduced tyrosine hydroxylase (TH) immunoreactivity, and behavioral abnormalities in rats. PTEN-induced kinase 1 (PINK1) is linked to recessive PD. Pink1 deletion results in impaired DA release and decreased mitochondrial respiration in the striatum of mice. Objective: To test if overexpression of PINK1 in C57BL/6 mice striatum may alleviate the injury of dopaminergic neuron caused by Rotenone. Methods: Lentivirus of GFP-PINK1-LV, GFP-PINK1^G309D-LV or GFP-vector-LV were injected into the C57BL/6 mice (male, 7 weeks old,18-20g weight) striatum. Two weeks later, Rotenone dissolved in DMSO was injected into the C57BL/6 mice lateral ventricles. After 2 weeks, TH protein level in C57BL/6 mice striatum was measured by Western blotting and Immunohistochemical staining. Meanwhile the behavioral performance was evaluated in Open Filed experment. Results: Western blotting and immunohistochemical experiments have proved that over-expression of wild-type PINK1 in C57BL/6 mice striatum may rescue the TH protein level reduction, but can not alleviate the behavioral injury caused by Rotenone.

Key words： PINK1(PTEN-induced kinase 1) Rotenone TH(Tyrosine hydroxylase)

Received: 08 October 2011 Published: 25 February 2012

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GONG Pu-sheng, ZHANG Jian-liang, FU Yue-jiao, JIA Huan-zhen, ZHAO Chun-li, LU Ling-ling, DUAN Chun-li, YANG Hui. Overexpression of PINK1 in C57BL/6 Mice Striatum May Alleviate Injury Caused by Rotenone. China Biotechnology, 2012, 32(02): 33-38.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I02/33

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