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| Cloning and Expression of S-mandelate Dehydrogenase Gene |
| ZENG Zhen, YANG Jun-fang, YANG Cheng-li, WANG Peng, LI Da-li |
| Department of Bioengineering, Nanjing University of Science and Technology, Nanjing 210094, China |
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Abstract S-mandelate dehydrogenase(SMDH) can catalyze S-mandelic acid to benzoylformic acid. The SMDH nucleotide gene(mdlA)was cloned from DNA of Pseudomonas putida NUST by PCR, and the amplicon was inserted into prokaryotic expression vector pET-30a (+). This recombinant was transformed into E. coli BL21(DE3) and then highly expressed by induction of IPTG. The result of SDS-PAGE showed that the molecular weight of cloned SMDH was about 43kDa. The recombinant strain could catalyze S-mandelate to benzoylformic acid.
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Received: 31 October 2011
Published: 25 February 2012
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