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| Comparison of celY Gene from Erwinia chrysanthemi Expression in Escherichia coli Driven by Different Regulatory Elements |
| CHEN Ke, HUANG Xiao, LU Lei, XING Yun, HOU Jing, WU Jie, LIU Jing-jing |
| State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China |
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Abstract Objective: The celY gene encoding endo-1,4-β-D-glucanase from a strain of Erwinia chrysanthemi was amplified by PCR and cloned into plasmid pUC19. To improve the expression and secretion of CelY, different regulatory elements were compared in driving the expression of CelY in Escherichia coli. Methods: A series of celY expression vectors containing different regulatory elements, such as lpp promoter, T7 promoter, pelB signal peptide, which were compared with the regulatory elements of celY, were constructed. Results: The series of celY expression vectors driven by lpp promoter, T7 promoter, pelB signal peptide, in varying degrees, increased the secretion expression level of CelY. Conclusion: The lpp promoter, T7 promoter, pelB signal peptide could be considered as candidates for the construct of a high-effective secretion vector.
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Received: 20 October 2011
Published: 25 February 2012
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