Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (10): 39-44    DOI:
    
Study on the Process of Enzymatic Hydrolysis of Fresh Antler Velvet
WANG Lan-lan, GAO Chao, ZHANG Long
Jilin Provincial Engineering Laboratory for the Complex Utilization of Biomass and Petrochemical Resources, Changchun University of Technology, Changchun 130012, China
Download: HTML   PDF(784KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

The process of Trypsase, Alcalase protease and Papain hydrolysis of fresh antler velvet was investigated. Alcalase protease showed the highest degradation efficiency. it was founded that enzymatic concentration, hydrolysis temperature, pH and hydrolysis time were the main parameters which affect the degradation yield of fresh velvet antler. by single factor experiments. The optimal hydrolysis conditions were obtained by the orthogonal tests as follows: substrate concentration 0.08 g/ml, enzymatic hydrolysis temperature 65 ℃, pH 9.0 and hydrolysis time 6.0 h. Under the conditions,the degradation yield of fresh antler velvet is up to 92.6%, and the yield of the amino acids product reached to 12.1%.



Key wordsFresh      antler velvet      Alcalase protease      Degradation yield      Amino acids      Technological conditions     
Received: 20 May 2011      Published: 25 October 2011
ZTFLH:  Q819  
Cite this article:

WANG Lan-lan, GAO Chao, ZHANG Long. Study on the Process of Enzymatic Hydrolysis of Fresh Antler Velvet. China Biotechnology, 2011, 31(10): 39-44.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I10/39


[1] 国家药典委员会.中华人民共和国药典(一部):北京化学工业出版社,2000. 264-265. State Pharmacopoeia Commission. The Pharmacopoeia of the People's Republic of China. Beijing: The Press of Chemical Industry, 2000. 264-265.

[2] 董万超,赵景辉, 潘久如,等.梅花鹿七种产品的化学成分研究.特产研究,1994,(1):36-43. Dong W C,Zhao J H, Pan J R, et al. Chinese Science Wild Economic Animal and Plant Reseach,1994,(1):36-43.

[3] Shoaf C, Philips R D, Saalia F, et al.Isolation and characacterization of four peptides hydrolysates from the brush border of rat intestinal mucosa. Biochem Biophys Acta,1976,445:694-703.

[4] 张根生,周云, 费英敏,等.鸡全蛋蛋白质酶水解工艺的研究.食品工业科技,2003,24(1):55-57. Zhang G S, Zhou Y, Fei Y M, et al. Science and Technology of Food Industry, 2003,24(1):55-57.

[5] Schreiner E, Nair N N, Marx D,et al.Enhaned absorption of calcium by casein phosphopeptides in rachitic and mormal chicks.Nutr, 1980, 110:2141-2152.

[6] 李银清, 毕胜男, 韩烨,等. 醇提法制备鹿茸胶原的初步研究.特产研究,2007,(1):9-11. Li Y Q,Bi S N,Han Y, et al. Special Wild Economic Animal and Plant Research,2007,(1):9-11.

[7] 黄晓巍,于秀华,付春.鹿茸乙醇提取工艺的研究. 社区用药指导,2006,8:146. Huang X W,Yu X H,Fu C. The Guide Community Medication,2006,8:146.

[8] 徐桂英,徐元礼,刘玉华.梅花鹿鹿茸多糖优选提取工艺条件的研究. 河南畜牧兽医综合版,2007,28(2):9-10. Xu G Y,Xu Y L,Liu Y H, et al. Husbandry and Veterinary Comprehensive Edition of Henan,2007,28(2):9-10.

[9] 徐志红,李淑芬,王金宇.超临界CO2萃取鹿茸中的性激素研究. 中国中药杂志,2007,32(19):2000-2003. Xu Z H, Li S F, Wang J Y, et al. China Journal of Chinese Materia Medica,2007,32(19):2000-2003.

[10] 周冉,李淑芬. 环糊精饱和水溶液法脱除鹿茸超临界萃取物中胆固醇的研究..食品科学,2008,29(7):179-182. Zhou R,Li S F. Food Science,2008,29(7):179-182.

[11] 徐志红,王金宇,李淑芬.快速测定鹿茸超临界CO2萃取物中胆固醇的含量.中药材,2006,29(8):800-802. Xu Z H,Wang J Y, Li S F, et al. Journal of Chinese Medicinal Materials,2006,29(8):800-802.

[12] 方海红,胡好远,黄红英,等.微生物碱性蛋白酶的研究进展.微生物学通报,2002,29(2):57-59. Fang H H,Hu H Y,Huang H Y, et al. Microbiology Bulletin,2002,29(2):57-59.

[13] 邵伟,乐超银,陈菽.大豆多肽Alcalase 酶解法制备工艺研究及应用.中国酿造,2008,(15):61-71. Shao W, Yue C Y, Chen S, et al. China Brewing,2008,(15):61-71.

[14] 吴建中,赵谋明,宁正祥.双酶法生产低苦味大豆多肽研究.粮油加工与食品机械,2003,4:24-27. Wu J Z,Zhao M M, Nin Z X. Science and Technology of Food Industry,2003,4:24-27.

[15] María M Yust, Justo P. Production of ace inhibitory peptides by digestion of chickpea legumin with alcalase. Food Chemistry, 2003, 81(3):363-369.

[16] 刘宁,仇农学.碱性蛋白酶Alcalase水解杏仁蛋白制备ACE抑制肽.产品加工,2009,149-152. Liu N, Qiu N X. Academic Periodical of Farm Products Processing,2009,149-152.

[17] 陶谦.酶水解法制取食用花生蛋白的研究.食品与发酵工业,1998,24(6):1-5. Tao Q. Food and Fermentation Industry,1998,24(6):1-5.

[18] 屠春燕,袁艳娟,徐娟等.Alcalase 碱性蛋白酶酶解绿豆分离蛋白制备小分子肽的工艺研究.食品研究与开发,2009,30(2):23-27. Tu C Y, Yuan Y J, Xu J, et al. Food Research and Development,2009,30(2):23-27.

[1] HUANG Xiang-feng, WANG Yi-han, LIU Jia-nan, LIU Jia, LU Li-jun. Research Progress in the Impacts of Promoting Factors on Biosurfactants Synthesis[J]. China Biotechnology, 2014, 34(7): 81-88.
[2] LU Ming-feng, KANG Shou-kai, ZHANG Yue-jie, ZHANG Hong-yu. Construction of a DNA Library in Small Cassette Encoding Random Peptide Consisting of 15 Amino Acids[J]. China Biotechnology, 2012, 32(02): 69-75.