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Preparation and Method of Microcarrier Based on Konjac Glucomannan Microsphere |
KANG Ji-yao1,2, ZHANG Ning1,2, ZHOU Wei-qing2, SUN Li-jing2, ZHANG Gui-feng2, MA Guang-hui2, SU Zhi-guo2 |
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; 2. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing 100190, China |
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Abstract Konjac glucomannan (KGM) microspheres were activated with 1,4-butanediol diglycidyl ether, and the collagen was coupled on the activated microsphere for preparation of micro-carrier for cell culture. For process optimization, the orthogonal regression experiments were carried out by four factors and three levels including activation time, protein consumption, coupled time, the crosslinker dosage effect of microcarrier cell culture. Based on culture effect of Vero cell, the optimum condition for preparation of collagen coated micro-carrier is activation time 5 h, the coupling of time 5 h, protein dosage 1 g:0.1 g (ball:protein), the amount of crosslinking agent 1g: 0.5ml (bead:crosslinking agent). The largest cell density was 1.7?106 cells/ml under the optimal preparation condition. The result indicates that KGM microspheres coupled with collagen is suitable for cell culture as microcarrier.
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Received: 25 February 2013
Published: 25 May 2013
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