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| Optimization of Fermentation Process for Gibberellic Acid Production Coupling with Adsorptive Extraction by Macroporous Resin |
| WANG Wei, LI Zhong-hai, LI Ji-li, ZENG Bo-quan |
| College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China |
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Abstract The mechanism of end product inhibition exists in gibberellin acid (GA3) biosynthesis of mycelium of Gibberella fujikuroi. Fermentation technology coupling with resin extraction would decrease the product inhibition and enhance the efficiency of GA3 production. Four macroporous resins were tested for their static adsorption/desorption performance towards GA3, and D-100 resin was selected as adsorbent through the method compared the adsorption capacity of the GA3 and elution efficiency. The facts about addition time and the amount of resin were studied by regression and response surface analysis. The experimental results showed that the optimal addition time and amount of resin were 70.75h and 2.02%, accordingly. The total gibberellin productivity and average specific production rate in the fermentation was 221.75mg and 0.96mgGA3/(gBiomass穐), respectively. Using the coupling technology in the fermentation, the gibberellin production increased by 149.6% compared with controls without D-100 resin.
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Received: 03 May 2012
Published: 25 November 2012
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