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| Cloning and Transient Expression of the Three Stress-related Rice Promoters |
| SHEN Qiu-shuo, CHEN Feng, YE Qing, LI Tao, CHEN Xin-bo, ZHANG Xian-wen |
| College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China |
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Abstract In order to clarify stress-inducible rice promoter function, the three heat- and salinity-induced genes (Os11g0453900, Os09g0526600, Os05g0381400) were picked out based on our previous microarray data of rice panicle and GEO database query. According to the bioinformatics analysis on the 1.5kb promoter sequences upstream of ATG, the 3 promoter fragments comprising specific cis-elements responsive to ABA, high temperature, high salinity were cloned, and named after Rab16Dp、OsHsfB2cp and PM19p, respectively. The results of Agrobacterium-mediated transient expression showed that Rab16Dp and OsHsfB2cp exhibited obvious activity to drive GUS gene expression under high temperature and salinity, but PM19p had no detectable activity under these conditions. The present work set the seal on the further research on the three promoters, and will provide important clues for the development of stress-inducible rice promoters.
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Received: 13 August 2012
Published: 25 November 2012
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