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Effect of Low Expression of CENP-E on Breast Cancer Cell Line MCF-7 |
ZHAN Xiao-qin, HU Min, SONG Pei-pei, ZHANG Fan, LIU Chen, SHI Qiong, WENG Ya-guang |
Key Laboratory for Clinical Laboratory Diagnostics of Education Ministry, College of Laboratory Medical, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective To explore the role of mitotic protein CENP-E in cancer.Methods Plasmid vector containing CENP-E special short hair RNA(shRNA)was transfected into human breast cancer MCF-7 cells.Nest PCR and Western blot was used to detect CENP-E mRNA and protein level;the proliferation of cells with down-regulated CENP-E were analyzed by MTT assay;the apoptosis of cells were detected by flow cytometry(FCM);the migration and invasion ability of cells were evaluated by transwell assay;indirect immunofluorescence was used to detect CENP-E protein and cell mitosis.Results Compared with the control group,the CENP-E mRNA and protein level in MCF-7 cells transfected shRNA-CENP-E were decreased; the cell growth were inhibited in shRNA-CENP-E group(P<0.05);the results of FCM showed that the cell apoptosis ratio were obviously increased after down-regulated CENP-E;indirect immunofluorescence indicated CENP-E protein was reduced after transfected with shRNA-CENP-E,and the nucleus showed abnormal caryogram;but the cell ability of migration and invasion in shRNA-CENP-E group were increased(P<0.05). Conclusion: Down-regulated part of CENP-E gene expression in breast cancer MCF-7 cells can inhibit cell proliferation,induce cell apoptosis,meanwhile enhance the cell ability of migration and invasion.
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Received: 19 July 2012
Published: 25 October 2012
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