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| Micropropagation of Pinellia Ternata (Thunb.) Breit in a Bioreactor Using Temporary Immersion System |
| JIA Ming-liang1,2, ZHANG Ben-hou2, GAO Wei-ping2, CHEN Ji-shuang1,2, OUYANG Ping-kai1 |
1. Institute of Bioresourse Engineering, Nanjing University of Technology, Nanjing 210009, China;
2. Dafeng Marine Industrial Institute, Nanjing University of Technology, Dafeng 224145, China |
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Abstract Objactive: To improve a new system for micropropagation of Pinellia ternata (Thunb.) Breit. in a temporary immersion bioreactor. Methods: Average number of tubers per plantlet (NTPs), Fresh weight (FW), Average fresh weight per plantlet (FWPs), Dry weight/Fresh weight (DW/FW) and the status of plantlet were studied refering standards. Firstly, leaves, petioles and cluster buds were cultured to establish the best explants type in the temporary immersion system. Secondly, petioles were cultured in the temporary immersion system, solid and liquid culture methods to compare the training effect. Results: Different types of explants were cultured in this temporary immersion system. Highest NTPs of 39.15 was achieved by culturing cluster bud. The petiole was 27.65 and the leaf was 18.05. Fresh weight has the same rule with NTPs. FWPs and DW/FW both have no significant differences. The plantlets induced by petioles were the most robust and which were more in thin induced by cluster buds. NTPs induced by petioles were achieved by different culture methods were as follows: temporary immersion system was 24.73, solid culture method was 14.75 and liquid culture method was 12.26. FWPs and DW/FW of temporary immersion system were optimal. The solid method and temporary immersion system were the suitable culturing methods for. The status of plantlets cultured by solid method was better than temporary immersion system. And the plantlets of temporary immersion system were thinner. Length of stoma guard cell (LSGC), Width of stoma guard cell (WSGC) and Average number of chloroplast per stoma guard cell (NCPs) have no significant difference between the two methods. The vitrification of plantlets cultured by liquid method has less number of chloroplasts, so the liquid method was not suitable for cultivation of Pinellia ternata (Thunb.) Breit. Conclusion: Petioles and cluster buds are suitable for the temporary immersion culture, and the petiole is better. Compared with conventional solid and liquid culture, temporary immersion culture system behaved as the best in various physiological indexes and that this system has the potential of large-scale micropropagation for this medicinal plant.
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Received: 19 September 2012
Published: 25 November 2012
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