| 研究简报 |
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| The procaryotic expression, purification and activity analysis of VIP-sTNFRⅡ |
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Abstract A prokaryotic expression plasmid containing VIP (vasoactive intestinal peptide) and sTNFRⅡ(soluble tumor necrosis factor receptor II ) genes was constructed. The sTNFRⅡ was cloned by PCR by using special primers which contained VIP gene ORF and a linker in its forward primer. The amplified fragment was inserted into the expression vector pET32a between Bam HI and Hind Ⅲ restriction sites. Transformed E coli DH5 by pET32a-VIP- sTNFRⅡexpressed the fusion protein. After being identified, the protein was purified by ion exchange chromatography and by hydrophobic interaction chromatography. The reconstructed protein showed high bio-activity and could be applied for further use.
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Received: 06 March 2006
Published: 25 May 2006
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