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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (4): 74-79    DOI: 10.13523/j.cb.20150411
    
Generation of FBXL15 Conditional Knockout Mice Using the Loxp-Cre Strategy
CHEN Jing-jing1,2, XING Gui-chun2, ZHANG Ling-qiang1,2
1. Graduate School, Anhui Medical University, Hefei 230032, China;
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 100850, China
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Abstract  

To generate F-box and Leucine-rich repeat protein 15 conditional knockout mice model,and confirm the genotype for further functional research. Using KO first strategy,to construct the conditional knockout vector targeting exon2 and exon3.In additional, FRT-SA-IRES-lacZ-loxp-neo-loxp element between intron 3 and intron 4 was also inserted.By Long Range PCR and Southern blot methods the positive clones were selected. Therefore the dual selected ES cells were microinjected into blastula of C57BL/6J mice, then blastula transplantations into the host mice,Chimerical mice were generated and the FBXL15-Lox Ptargeted mice were identified by PCR method. This animal model established the foundation for further study of FBXL15's pivotal roles in regulation upon embryos development and bone metabolism



Key wordsFBXL15 gene      Conditional mouse model      ES cell      Embryos development      Bone metabolism     
Received: 16 January 2015      Published: 25 April 2015
ZTFLH:  Q789  
Cite this article:

CHEN Jing-jing, XING Gui-chun, ZHANG Ling-qiang. Generation of FBXL15 Conditional Knockout Mice Using the Loxp-Cre Strategy. China Biotechnology, 2015, 35(4): 74-79.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150411     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I4/74


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