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Production of the Isoamyl Alcohol in E.coli by Expression of kivD Gene |
XIAO Shi-yuan1,2, XU Jing-liang1, CHEN Xiao-yan1, YANG liu1, YUAN Zhen-hong1 |
Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China |
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Abstract Keto acid decarboxylase is the key enzyme for biological synthesis of isoamyl alcohol, rarely existed in E. coli. Keto acid decarboxylase gene kivD(rbs) obtained from Lactococcuslactis subsp.Lactis genome DNA through reaction of PCR, was inserted into E. coli high expression vector pET-28a(+). The formed pET-kivD(rbs), was then transformed into E. coli BL21(DE3) by hot blow. The activity of keto acid decarboxylase and isoamyl alcohol were detected in the fermentation broth.
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Received: 14 January 2015
Published: 25 April 2015
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