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Relationship between the Bioactivity of BMP2/7 Heterodimers and its Regulation of CIZ Expression |
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Abstract Abstract BMP2/7 heterodimer has stronger bioactivity than BMP2 homodimer, but the mechanism remains to be unclear. Treating the osteoblast MC3T3-E1 cells with BMP proteins, results of Alkalin phosphatase staining , Alizarin red staining , as well as p3GC2-LUX luciferase assay demonstrated that BMP2/7 heterodimers significantly promoted osteogenic differentiation to a higher extent than BMP2 homodimers. Overexpression of CIZ in MC3T3-E1 significantly inhibited the promotion of ALP and Osteocalcin expression by BMP2/7, and blocked activation of BMP/Smad. BMP protein may induce the expression of CIZ, but effects of BMP2/7 heterodimer is weaker than BMP2 homodimer. It suggested that the effects of BMP2/7 on CIZ expression is related to its bioactivity.
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Received: 16 May 2007
Published: 25 September 2007
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