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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (3): 61-65    DOI: 10.13523/j.cb.20150309
    
Preparation of Recombinant PACAP 27-derived Peptide RP2 and Its Effects on Promoting Corneal Epithelial Cell Proliferation
WANG Dong-Bo, MA Yi, WANG Xiao-Li, ZHAO Shao-Jun, HAN Lei, HONG An
Department of Cell Biology of Jinan University, Institute of Biological Medicine of Jinan University, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China
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Abstract  

PACAP 27-derived peptide RP2 which can promote promote the epithelial cell proliferation is efficiently producted by genetic engineering and be used to study its effects on corneal epithelial cells,which can provide an experimental basis for the treatment for corneal injury. Recombinant peptide RP2 was expressed by genetic recombinant technology after the prokaryotic expression, purification by Chitin-Beads column, and producted,analyzed、identification by HPLC, SDS-PAGE and mass spectrometry, then its effects on mouse corneal proliferation were studied. Experimental results showed that the molecular weight of RP2 is 3.3 kDa. and the purity is 96%. Mouse corneal epithelial cells were respectively treated with recombinant peptide RP2、 PACAP 27 and PBS for 24 h and 48 h,The proliferation rate of mouse corneal epithelial cells treated with recombinant peptide RP2,is (49.6±3.1)% and(93.0±1.7)%, the PACAP 27 group is (29.0±2.4)% and (78.8±2.6)%,while the PBS group is (20.2±1.1)% and (40.9±3.3)%. Therefore, the producted recombinant peptide RP2 can significantly stimulate corneal epithelial cell proliferation, so it will likely to become a new candidate drug for corneal injury.



Key wordsGene recombination      Pituitary adenylate cyclase-activating polypeptide (PACAP)      Recombinant peptide      Corneal epithelial cells     
Received: 28 January 2015      Published: 25 March 2015
ZTFLH:  Q819  
Cite this article:

WANG Dong-Bo, MA Yi, WANG Xiao-Li, ZHAO Shao-Jun, HAN Lei, HONG An. Preparation of Recombinant PACAP 27-derived Peptide RP2 and Its Effects on Promoting Corneal Epithelial Cell Proliferation. China Biotechnology, 2015, 35(3): 61-65.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150309     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I3/61


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