Expression and Antiviral Effects of <em>Sus Scrofa</em> Mx1 and <em>Bos taurus</em> Mx1 on PRV

doi:10.13523/j.cb.20150301

China Biotechnology

2015, Vol. 35

Issue (3): 1-7 DOI: 10.13523/j.cb.20150301

Expression and Antiviral Effects of Sus Scrofa Mx1 and Bos taurus Mx1 on PRV

WANG Peng, ZHENG Zhao-xin, LIU Ming-qiu

School of Life Sciences, Fudan University, Shanghai Engineering Research Center of Industrial Microorganisms, Shanghai 200433, China

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Abstract

Objective: To study the ability of Sus Mx1 and Bos Mx1 to inhibit the replication of PRV. Methods: Sus scrofa Mx1 gene and Bos taurus Mx1 gene were cloned from IBRS-2 and MDBK cells to pcDNA3.1/myc-His (-) B vector respectively, and transfected into PK-15 cells with Lipofectamine ^TM2000 to detect whether they can express successfully in cells by RT-PCR and Western blot respectively. And then the cytotoxicity to cells WST-1 and the ability of Sus Mx1 and Bos Mx1 to inhibit the replication of PRV were checked. The PK-15 cells were infected with 20TCID₅₀, 100TCID₅₀PRV after transfection 24h. Subsequently, samples were collected after infection 24h, 48h, 72h, and total viral copies were tested by extracting RNAs of frozen and thawed samples repeatedly. Meantime, the cytopathic effect after infecting 100TCID₅₀PRV in 72h was observed. Results: both recombinant eukaryotic plasmids were constructed and expressed successfully. They were able to reduce the amount of RNA of PRV significantly by real time PCR (P <0.01). Conclusion: Sus scrofa Mx1 and Bos taurus Mx1 can inhibit the replication of PRV in PK-15.

Key words： Sus scrofa Mx1 Bos taurus Mx1 PK-15 cells PRV

Received: 11 December 2014 Published: 25 March 2015

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WANG Peng, ZHENG Zhao-xin, LIU Ming-qiu. Expression and Antiviral Effects of Sus Scrofa Mx1 and Bos taurus Mx1 on PRV. China Biotechnology, 2015, 35(3): 1-7.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150301 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I3/1

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