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The Breeding of High-yielding Actinoplanes of Rapamycin |
SHI Man-man1, QIAO Chang-sheng1,2, ZHU Ming1, LI Xue2, LIU Shan-shan1 |
1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Peiyang Biotrans Co., Ltd, Tianjin 300457, China |
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Abstract The Actinoplanes of BCLP-016 was selected as the original strain, and a mutagenesis of atmospheric and room temperature plasma (ARTP) was used to treat its spores, the spore suspension treated with three different time was mixed. After dilution coated, picking some single colonies according to the colony morphology of the strain for screening, after fermentation rescreening, a high-yielding strain of rapamycinARTP-039 was screened out, its rapamycin production can reach 369.39mg/L, compared with the yield of the original strain BCLP-016 256.86 mg/L, increased by 43.81%.Choosing the high-yielding strain ARTP-039 as the starting strain, the traditional UV mutagenesis was conducted, three time that corresponding with fatality rate was selected to treat its spore suspension, based on the theory of the ribosome engineering, picking out five kinds of resistant material of streptomycin, gentamicin, rifampicin, chloramphenicol and erythromycin to conduct the resistance screening. After fermentation rescreening, a high-yielding strain of St8+Gen6+Rif9+Chl3+Er4-015 was screened out, it has five kinds of resistance. The strain fermentation test showed that after 7 days of fermentation, the rapamycin output can reach 589.79mg/L, compared with the original strain BCLP-016 yield increased by 129.61%,and its genetic stability is good.
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Received: 22 October 2014
Published: 25 February 2015
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[1] 杨冬.雷帕霉素高产菌株的选育.杭州:浙江大学,材料与化工学院,2006. Yang D.Breeding of rapamycin high-production strains.Hangzhou:Zhejiang University,College of Material Science and Chemical Engineering,2006.
[2] Balter M.Protein matchmaker may lead new gene therapy to the altar.Science,1996,273(5272):183.
[3] Hidalgo M,Rowinsky E K.The rapamycin-sensitive signal transduction pathway as a target for cancer therapy.Oncogene,2000,19(56):6680-6686.
[4] Li H P,Li G,Sun W T,et al.Radio-frequency,atmospheric-pressure glow discharges:producing methods,characteristics and applications in bio-medical fields.AIP Conference Proceedings,2008,982(1):584-591.
[5] Li G,Li H P,Wang L Y,et al.Genetic effects of radio-frequency,atmospheric-pressure glow discharges with helium.Applied Physics Letters,2008,92(22):221504.
[6] Wang L Y,Huang Z L,Li G,et al.Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma.Journal of Applied Microbiology,2010,108(3):851-858.
[7] Shi J J,Kong M G.Expansion of the plasma stability range in radio-frequency atmospheric-pressure glow discharges.Applied Physics Letters,2005,87(20):201501.
[8] 施巧琴,吴松刚.工业微生物育种学.第2版.北京:科学出版社,2003.201-248. Shi Q Q,Wu S G.Industrial Microbiology Breeding.2nded.Beijing:Science Press,2003.201-248.
[9] 陈红歌,刘新育,张世敏,等.木聚糖酶高产菌株的诱变.微生物学通报,2004,31(6):33-36. Chen H G,Liu X Y,Zhang S M,et al.Mutation of the strain producing higher xylanase.Microbiology China,2004,31(6):33-36.
[10] 杜海英,于宏伟,韩军,等.原生质体诱变选育乳糖酶高产菌株.微生物学通报,2006,33(6):48-51. Du H Y,Yu H W,Han J,et al.Breeding of high-yielding β-galactosidase strains from protoplast of Aspergillus niger.Microbiology China,2006,33(6):48-51.
[11] Ochi K.From microbial differentiation to ribosome engineering.Bioscience Biotechnology and Biochemistry,2007,71(6):1373-1386.
[12] 谢庶洁,肖静,徐俊.微生物核糖体工程研究进展.微生物学报,2009,49(08):981-986. Xie S J,Xiao J,Xu J.Research progress of microbial ribosome engineering.Acta Microbiologica Sinica,2009,49(8):981-986.
[13] 陈丽仙.核糖体工程技术在刺糖多孢菌上的初步应用.福州:福建农林大学,生物学院,2010. Chen L X.Preliminary application of ribosome engineering technology in Saccharopolyspora spinosa.Fuzhou:Fujian Agriculture and Forestry University,College of Biological Sciences,2010.
[14] Kojima N,Kojima Y,Sakakibara T.Novel rapamycin producer.European:001534,1993-11-11.
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