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| Functional expression of human cytochrome P450 enzymes in escherichia coli |
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Abstract Abstract Functional expression of human cytochrome P450（P450，CYP）in E.coli is important for new drug R&D, clinical drug therapy and the study of early ADME/T properties. Escherichia coli is the most extensively utilized host in the production of recombinant human P450 enzymes. However, it is challenging to obtain sufficient P450s with catalytic activity.Depict current developments in the heterologous expression of human P450 enzymes, strategies for the expression of human P450s in E.coli, factors affecting highlevel expression, and coexpression. The recent works of authors in expression optimization are also reviewed.
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Received: 17 September 2008
Published: 28 July 2009
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