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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (5): 81-85    DOI:
    
Study on the Conditions of Electrotransformation in Saccharopolyspora spinosa
ZHANG Qiu-xue1,2, LAN Zhou2, WANG Yang2, WANG Chang-lu1, SONG Yuan3, ZHANG Xiao-lin2
1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Academy of State Administration of Grain, Beijing 100037, China;
3. College of Biological Sciences, China Agricultural University, Beijing 100094,China
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Abstract  

Saccharopolyspora spinosa CB11was used as the recipient strain . Optimal conditions including growth stage of the strain, electroshock voltage,DNA concentration were investigated for the electrotransformation of CB11 with Escherichia coli-Streptomyces shuttle vector pSET152.It was showed that the highest electroporation efficiency was yielded under the cluture age of 48h, electric field strength of 12kV/cm and minimum DNA concentration needed 0.1μg . Plasmid stability experiment and PCR of acc(3)IV gene showed that pSET152 was successfully electroporated into and could stably exist in the Saccharopolyspora spinosa CB11. This protocol would be useful for genetic studies of S. spinosa.



Key wordsSaccharopolyspora spinosa      Electroporation      Plasmid pSET152     
Received: 20 January 2011      Published: 27 May 2011
ZTFLH:  Q78  
Cite this article:

ZHANG Qiu-xue, LAN Zhou, WANG Yang, WANG Chang-lu, SONG Yuan, ZHANG Xiao-lin. Study on the Conditions of Electrotransformation in Saccharopolyspora spinosa. China Biotechnology, 2011, 31(5): 81-85.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I5/81


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