[1] Hube F, Reverdiau P, Iochmann S,et al. Improved PCR method for amplification of GCrich DNA sequences.Mol Biotechnol,2005,31(1):81~4
[2] 胡福泉.现代基因操作技术.北京:人民军医出版社,2000
Hu F Q.Modern Gene Technology.Beijing:People’s Militany Medical Press,2000
[3] Mullis K B, Faloona F A. Specific synthesis of DNA in vitro via a polymerasecatalyzed chain reaction. Methods Enzymol, 1987, 155(56):355~350
[4] Bachmann H S,Siffert W,Frey U H. Successful amplification of extremely GCrich promoter regions using a novel 'slowdown PCR' technique. Pharmacogenetics. 2003,13(12):759~66
[5] Moreau A, Wang D S, Forget S,et al. GCrich template amplification by inverse PCR,DNA polymerase and solvent effects. Methods Mol Biol,2002(192):75~80
[6] Innis M A,Gelfand D H,Sninsky J J, et al. PCR protocols:a guide to methods and applications. San Diego CA:Academic Press,1990,22(4)∶3~7
[7] Erlich H A,Gelfand D,Sninsky J J.Recent advances in the polyrnerase chain reaction. Science,1991,(252):1643~1651
[8] 刑小黑,姜卫红.提高富含GC碱基DNA模板PCR扩增效率的方法.南京农业大学学报,1999,22(3):112~114
Xing X H,Jiang W H.Journal of Nanjin Agriculturd University,1999,22(3):112~114
[9] Sheffield V C, Beck J S, Stone E M,et al. A simple and efficient method for attachment of a 40base pair, GCrich sequence to PCRamplified DNA. Biotechniques. 1992,12(3):386~388
[10] Turner S L, Jenkins F J. Use of deoxyinosine in PCR to improve amplification of GCrich DNA. Biotechniques,1995,19(1):48~52
[11] Sun Y, Hegamyer G, Colburn N H. PCRdirect sequencing of a GCrich region by inclusion of 10% DMSO: application to mouse cjun. Biotechniques,1993,15(3):372~374
[12] Barnes W M. PCR amplification of up to 35kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc Natl Acad Sci USA,1994,91(6):2216~2200
[13] Miller G A. Method for nucleotide sequence amplification,United States Patent, 1996, (5):545~539
[14] Cheng S, Fockler C, Barnes W M,et al. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci USA,1994,91(12):5695~5699
[15] Sahdev S, Saini S,et al. Ampification of GCrich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions. Molecular and cellular Probes,2007, 21(11):303~307
[16] Nu Y,Stulp R P.Improved mutation detection in GCrich DNA fragment by combined DGGE and CDGE. Nucleic Acids Res, 1999,27(15):e9
[17] Henke W, Herdel K,Jung K,et al. Betaine improves the PCR amplification of GCrich DNA sequences. Nucleic Acids Res, 1997,25(19):3957~3958
[18] 陈绪清,张晓东.甜菜碱增强长片段PCR的扩增.生物工程学报,2004,20(5):717~718
Chen X Q,Zhang X D.Chinese Journal of Biotechnology,2004,20(5):717~718
[19] Baskaran N,Kandpal R P,Bhargava A K,et al.Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Genome Res,1996,6(7):633~638
[20] 张治洲,杨霞,刘芳,等.有机试剂增强高GC含量DNA片段扩增效率的方法:中国,200810151276.4
|