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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 69-73    DOI:
    
Ethylene glycol and 1,2-propanediol enhanced polymerase chain reaction for amplification of high GC content human DNA
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Abstract  

Investigators in gene (genome) manipulation frequently meet difficulty in amplification of GC-rich DNA sequences. This difficulty has not been well solved worldwide. Through systematic screening, 1,2-propanediol and ethylene glycol were found specifically efficient in enhancing the amplification of high GC content human genomic DNA. We randomly selected 104 GC-rich sequences (GC content from 60% to 80% and length from 700 to 800bp) from human genomic DNA to test the two organic solvents and all 104 amplicons were well amplified.



Key wordsGC-rich;Ethylene glycol;1,2-propanediol;PCR     
Received: 24 December 2008      Published: 31 March 2009
ZTFLH:  Q75  
Cite this article:

YANG Xia- Liu-Fang- Meng-Liang-Yu- Zhang-Chi-Zhou. Ethylene glycol and 1,2-propanediol enhanced polymerase chain reaction for amplification of high GC content human DNA. China Biotechnology, 2009, 29(03): 69-73.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/69

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