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| Prokaryotic Expression of Soluble Ectodomain of Human Fibroblast Growth Factor Receptor 2IIIc and Its Inhibitory Effect on the Proliferation of DU145 Cells |
| HE Ying1, WANG Ju1,2, ZHANG Zhi-cheng1, LIU Xue-ting1, HONG An1,2 |
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Abstract Abstract The gene of soluble ectodomain of FGFR2IIIc (sFGFR2IIIc) was cloned into pET3c, and expressed as inclusion bodies in E.coli. The inclusion bodies were refolded by dialysis and then purified. The purity of sFGFR2IIIc was more than 95% after heparin affinity chromatography. The result of co-IP indicated that the product was able to bind FGF2, and the proliferation of prostate cancer DU145 cells line was inhibited by sFGFR2IIIc in a time-dependent and dose-dependent manner by MTT assay. Moreover, the phosphorylation of FGFRs on the cell membrane could be downregulated dramatically after treatment with sFGFR2IIIc. The results suggested that sFGFR2IIIc could inhibit the proliferation of DU145 cells through blocking the cell signaling of FGFs.
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Received: 22 December 2008
Published: 28 July 2009
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| ［1］ Eswarakumar V P,Lax I,Schlessinger J.Cellular signaling by fibroblast growth factor receptors. Cytokine & Growth Factor Reviews, 2005,16:139~149
［2］ Gowardhan B, West A F, Robson C N, et al. Adenovirus-mediated expression of a soluble fibroblast growth factor receptor inhibits in vitro growth of prostate DU145 cells. Prostate, 2004,61(1):50~59
［3］ Gowardhan B, Douglas D A, Mathers M E,et al. Evaluation of the fibroblast growth factor system as a potential target for therapy in human prostate cancer. British Journal of Cancer, 2005,92:320~327
［4］ Douglas F E, Karen A P, Alison M D, et al. Genome-wide association study identifies novel breast cancer susceptibility loci. Nature, 2007,447:1087~1095
［5］ Presta M, Dell′Era P, Mitola S, et al. Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis. Cytokine & Growth Factor Reviews, 2005,16:159~178
［6］ 赵嘉惠，张华屏，王春芳.MTT法在检测细胞增殖方面的探讨.山西医科大学学报，2007,38(3):262~263
Zhao J H, Zhang H P, Wang C F. J Shanxi Med Univ, 2007,38(3):262~263
［7］ Ueno H, Gunn M, Dell K, et al.A truncated form of fibroblast growth factor receptor 1 inhibits signal transduction by multiple types of fibroblast growth factor receptor. J Biol Chem, 1992,267:1470~1476
［8］ 方剑武，黄思罗，Michel Crepin,等.FGF-2/FGFR信号传导途径与肿瘤的关系及在乳腺癌发生中的作用. 科学通报，2003,48(13):1384~1392
Fang J W, Huang S L, Michel C, et al. Chinese Sci Bull, 2003,48(13):1384~1392
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